Hydrogen sulfide-induces DNA damage and changes in apoptotic gene expression in human lung fibroblast cells.
نویسندگان
چکیده
Hydrogen sulfide (H2S) has been shown previously to exert proapoptotic activity. However, the mechanism(s) by which H2S affects cell growth and function have not been addressed adequately. In this study, cultured human lung fibroblasts were treated with the H2S donor NaHS (10-75 microM; 12-48 h). NaHS caused a concentration-dependent increase in micronuclei formation (indicating DNA damage) and cell cycle arrest (G1 phase). NaHS increased expression of ku 70 and ku 80 but did not affect the expression of other DNA repair proteins such as proliferating cell nuclear antigen (PCNA) or replication protein A (rNase protection assay). NaHS treatment also resulted in stabilization of p53 coupled with induction of downstream proteins such as p21, Bax, and cytochrome c, as well as translocation of Bax from the cytosol to the mitochondria and release of cytochrome c from mitochondria. NaHS did not up-regulate cell levels of the antiapoptotic protein, Bcl-2. We propose that the genotoxic action of H2S propels the cell toward apoptotic death triggered initially by stabilization of p53 and subsequently involving a cascade of downstream products. These results are of significance as they uncover a hitherto unknown and very fundamental role for H2S in determining cell fate.
منابع مشابه
Gene expression changes of collagen І and ІІІ in human skin fibroblast cells in effect of microalga Chlorella vulgaris extract and compared to vitamin C
Skin aging is a biological process that is due to the reduction of collagen production and increase of multiple enzymes, including matrix metalloproteinase (MMPS), which degrade collagen. Chlorella vulgaris is a marine microalga and its beneficial effects on the skin make it a proper ingredient to be used in anti-aging products. In this study, the effect of C. vulgaris extract comparing to vita...
متن کاملThe Calotropis Gigantea Methanolic Extract Induces Apoptosis in Human Breast Carcinoma Cells
Background: Calotropis gigantea (family: Asclepiadaceae) has been known to contain cardiac glycosides. The C. gigantea extracts have been reported as cytotoxic to a few cancer cell lines. The present study was designed to examine the effect of Calotropis gigantea methanolic extract (CGME) on the growth and apoptosis in human breast carcinoma cell line (MCF-7 cells).Methods: The study was ...
متن کاملEffects of hydrogen peroxide-induced oxidative stress on the pattern of pro-apoptotic and anti-apoptotic genes expression during PC12 cells differentiation
Background and Objective: In neurodegenerative disorders, oxidative stress mediated by reactive oxygen species is strongly associated with increased neuronal damages that lead to apoptosis. Pro-apoptotic and anti-apoptotic gene expressions were changed during cell differentiation that affect cell viability and differentiation. This study was conducted to determine the effects of hydrogen peroxi...
متن کاملEffects of Hydrogen Peroxide Oxidative Stress on the Pattern of Pro-apoptotic and Anti-apoptotic Genes Expression During PC12 Cells Differentiation
Background and Aims:In neurodegenerative disorders,oxidative stress mediated by reactive oxygen species is strongly associated with increased neuronal damages which can lead to apoptosis. Pro-apoptotic and anti-apoptotic gene expressions are changed during the cell differentiation that affect cell viability and differentiation. Therefore, this study was conducted to determine the effects of hyd...
متن کاملEffect of trichostatin A on histone deacetylase 1 (HDAC 1) and CIP/KIP (p21CIP1/WAF1, p27KIP1, and p57KIP2) gene expression, cell growth inhibition and apoptosis induction in lung cancer COR-L105 cell line. Abstract
Effect of trichostatin A on histone deacetylase 1 (HDAC 1) and CIP/KIP (p21CIP1/WAF1, p27KIP1, and p57KIP2) gene expression, cell growth inhibition and apoptosis induction in lung cancer COR-L105 cell line. Abstract Background: Lung cancer is one the leading cause of cancer-related death worldwide, with more than 1.2 million deaths each year. In addition to genetic mutations, epigenetic modif...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology
دوره 21 1 شماره
صفحات -
تاریخ انتشار 2007